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KMID : 0879319931990010090
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Annuals of Clinical Study - Seoul National Hospital
1993 Volume.199 No. 1 p.90 ~ p.98
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Abstract
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This paper describes the stability of lysozyme chloride in phosphate buffer(PH 6.2) with micrococcus Lysodeikticus in suspension as a substrate. And the lysozyme chloride potencies in the Commercial lysozyme chloride preparations The activity was
decreased to about 97.5%, 84.1% and 71.7% after 1, 3, and 5 hrs. respectively in concentration of 0.4㎍/㎖ at 6℃. the activity was decreased to about 96.5%, 82.%, and 68.5% after 1, 3 and 5 hrs at a concentration of 0.4㎍/㎖ at 35℃ The
suspension
of
micrococcus lysodeikticus dried cells{50mg in 120㎖ of phosphate buffer(PH 6.2)} retained the original ability as substrate for 5hrs at 6℃, 5hrs at 25℃ and 1hr at 35℃The poteneies of A, B, C, D, E and F extracted with.
Phosphate buffer(PH 6. 2) were 83.4%, 105.9%, 102.6%, 91.6%, 29.7% hand 48.1% of labeled potencies, respectively. On the other hand, the potencies of 92.8%, 103.6%, 99.3%, 93.2%, 92.3% and 62.5% respectively. Were obtained when these preparations
were
extracted with phosphate buffer(PH 6.2) additionally containing 0.4M Nacl.
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